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znuA [2018-05-28 18:05:59]
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znuA [2018-05-28 18:05:59]

high-affinity ABC transporter for zinc (binding protein, lipoprotein)
locus
BSU02850
pI
5.15
mw
35.51 kDa
protein length
319 aa Sequence Blast
gene length
957 bp Sequence Blast
function
zinc uptake
product
ABC transporter for zinc (binding protein)
essential
no
synonyms
ycdH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.3|Zinc]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    308,332 → 309,291

    Phenotypes of a mutant

  • reduced genetic competence, can be rescued by the addition of excess zinc [Pubmed|21813502]
  • reduced expression of the ''[gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|yvyG]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]'' operon [Pubmed|21813502]

    • The protein

    Protein family

  • bacterial solute-binding protein 9 family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|3C40A6E42A669E77ADBF5FFF334061C51F76A4F9|MntA]

    • Structure

  • [PDB|2O1E]

    • [SW|Localization]

  • inner spore membrane [Pubmed|26731423]

    • Expression and Regulation

    Operons

    genes
    [gene|D33A144568CAF32FFE4A2A46BE67573DB66FC1A1|znuA]-[gene|6B5D4FD32CCE72C99B915B0F1EB4B8E8D42A04B3|znuC]-[gene|EE212953BC83BD6D69BF769D6E917F4F954BE1E2|znuB]
    description
    [Pubmed|9811636]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12426338], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur]: repression, in the presence of zinc [Pubmed|12426338], in [regulon|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|Zur regulon]

    • regulation

  • induced by zinc starvation ([protein|search|Zur]) [Pubmed|12426338]
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    Biological materials

    Mutant

  • BKE02850 (Δ[gene|D33A144568CAF32FFE4A2A46BE67573DB66FC1A1|znuA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGTATATCCCCTCTT, downstream forward: _UP4_TAAGATAGTGTGCGCCGGAG
  • BKK02850 (Δ[gene|D33A144568CAF32FFE4A2A46BE67573DB66FC1A1|znuA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCGTATATCCCCTCTT, downstream forward: _UP4_TAAGATAGTGTGCGCCGGAG

    • References

  • 12426338,9811636,10092453,21813502,12426338,18957862,18763711,26731423,27561249